Molecular characterization of two-component systems of Helicobacter pylori.

Biochemical characterization of protein complexes from the Helicobacter pylori protein interaction map: strategies for complex formation and evidence for novel interactions within type IV secretion systems.

We have investigated a large set of interactions from the Helicobacter pylori protein interaction map previously identified by high-throughput yeast two-hybrid (htY2H)-based methods. This study had two aims: i) to validate htY2H as a source of protein-protein interaction complexes for high-throughput biochemical and structural studies of the H. pylori interactome; and ii) to validate biochemica...

Molecular diagnosis of Helicobacter pylori in semen of infertile men

Molecular Detection of Oral Helicobacter pylori and Its Association with Dental Conditions Among Patients with Helicobacter Infection in Baghdad City

Background and Objective: Helicobacter pylori (H. pylori) is a bacterium known for its presence in the stomach, but it can also colonize the oral cavity and contribute to various oral and gastric diseases. This study aimed to detect the presence of oral H. pylori and investigate its potential association with dental caries and dental erosion. Methods: Saliva and plaque samples were collected f...

Molecular Identification of Helicobacter pylori and IceA Genes Frequency from Dental Plaques Isolated from People Using PCR Method

Background and Aims: Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. H. pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the pres...

Detection of Helicobacter Pylori Diagnostic Antigens in the Stool of Infected Patients

Background: Some antigens of H. pylori are excreted into the stool of infected people. These antigens can be used to detect the infection by immunoassays such as ELISA. Our aim was to identify these antigens by immunoblotting and affinity chromatography techniques. Methods: Four different antigenic preparations, namely, whole cell sonicate (WCS), outer membrane proteins (OMPs), cytoplasmic anti...